Anti-NELFA Antibody Picoband®

About this product

Boster Bio Anti-NELFA Antibody Picoband® catalog # A07040-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07040-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.

Gene Name

NELFA

Gene ID

7469

UniProt

Q9H3P2

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human NELFA recombinant protein (Position: I38-L482) . Human NELFA shares 94.6% amino acid (aa) sequence identity with mouse NELFA.

Clonality

Polyclonal

Applications

WB, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Narita, T., Yamaguchi, Y., Yano, K., Sugimoto, S., Chanarat, S., Wada, T., Kim, D., Hasegawa, J., Omori, M., Inukai, N., Endoh, M., Yamada, T., Handa, H. Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex. Molec. Cell. Biol. 23: 1863-1873, 2003. 2. Narita, T., Yung, T. M. C., Yamamoto, J., Tsuboi, Y., Tanabe, H., Tanaka, K., Yamaguchi, Y., Handa, H. NELF interacts with CBC and participates in 3-prime end processing of replication-dependent histone mRNAs. Molec. Cell 26: 349-365, 2007. 3. Wright, T. J., Costa, J. L., Naranjo, C., Francis-West, P., Altherr, M. R. Comparative analysis of a novel gene from the Wolf-Hirschhorn/Pitt-Rogers-Danks syndrome critical region. Genomics 59: 203-212, 1999.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

57 kDa

Applications Notes

6

Isotype

IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.