Anti-NR1H3 Antibody Picoband®

About this product

Boster Bio Anti-NR1H3 Antibody Picoband® catalog # A03331-3. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03331-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

LXRA is a tissue-specific cofactor that permits RXRA to function as a potent 9cRA receptor with a distinct target gene specificity. It specifically interacts with RXRA in vivo to form a functional heterodimer in which RXRA is the ligand-binding subunit. Additionally, LXR activity is critical for physiologic lipid metabolism and transport. LXRs are endogenous inhibitors of atherogenesis and are targets for therapeutic intervention in cardiovascular disease. Furthermore, LXRs and their ligands are negative regulators of macrophage inflammatory gene expression. LXR is also found that as a transcriptional switch that integrates hepatic glucose metabolism and fatty acid synthesis. The LXR-IDOL-LDLR axis defines a complementary pathway to sterol response element-binding proteins for sterol regulation of cholesterol uptake.

Gene Name

NR1H3

Gene ID

10062

UniProt

Q13133

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human NR1H3 recombinant protein (Position: D11-R401) . Human NR1H3 shares 91.3% and 90.5% amino acid (aa) sequence identity with mouse and rat NR1H3, respectively.

Clonality

Polyclonal

Applications

WB, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Willy, P. J.; Umesono, K.; Ong, E. S.; Evans, R. M.; Heyman, R. A.; Mangelsdorf, D. J. : LXR, a nuclear receptor that defines a distinct retinoid response pathway. Genes Dev. 9: 1033-1045, 1995. 2. Tangirala, R. K.; Bischoff, E. D.; Joseph, S. B.; Wagner, B. L.; Walczak, R.; Laffitte, B. A.; Daige, C. L.; Thomas, D.; Heyman, R. A.; Mangelsdorf, D. J.; Wang, X.; Lusis, A. J.; Tontonoz, P.; Schulman, I. G. : Identification of macrophage liver X receptors as inhibitors of atherosclerosis. Proc. Nat. Acad. Sci. 99: 11896-11901, 2002. 3. Joseph, S. B.; Castrillo, A.; Laffitte, B. A.; Mangelsdorf, D. J.; Tontonoz, P. : Reciprocal regulation of inflammation and lipid metabolism by liver X receptors. Nature Med. 9: 213-219, 2003. 4. Mitro, N.; Mak, P. A.; Vargas, L.; Godio, C.; Hampton, E.; Molteni, V.; Kreusch, A.; Saez, E. : The nuclear receptor LXR is a glucose sensor. Nature 445: 219-223, 2007. 5. Zelcer, N.; Hong, C.; Boyadjian, R.; Tontonoz, P. : LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor. Science 325: 100-104, 2009.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

50 kDa

Applications Notes

6

Isotype

IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.