Anti-CLCN3 Antibody Picoband®

About this product

Boster Bio Anti-CLCN3 Antibody Picoband® catalog # A05300-1. Tested in WB, IF, IHC, ICC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05300-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

H+/Cl? exchange transporter 3 is a protein that in humans is encoded by the CLCN3 gene. This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA) -activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca (2+) /calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Gene Name

CLCN3

Gene ID

1182

UniProt

P51790

Host

Rabbit

Reactivity

Human, Rat

Immunogen

E.coli-derived human CLCN3 recombinant protein (Position: G293-R752) . Human CLCN3 shares 99.6% amino acid (aa) sequence identity with mouse CLCN3.

Clonality

Polyclonal

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Borsani, G., Rugarli, E. I., Taglialatela, M., Wong, C., Ballabio, A. Characterization of a human and murine gene (CLCN3) sharing similarities to voltage-gated chloride channels and to a yeast integral membrane protein. Genomics 27: 131-141, 1995. 2. Duncan, A. R., Polovitskaya, M. M., Gaitan-Penas, H., Bertelli, S., VanNoy, G. E., Grant, P. E., O'Donnell-Luria, A., Valivullah, Z., Lovgren, A. K., England, E. M., Agolini, E., Madden, J. A., and 28 others. Unique variants in CLCN3, encoding an endosomal anion/proton exchanger, underlie a spectrum of neurodevelopmental disorders. Am. J. Hum. Genet. 108: 1450-1465, 2021. 3. Mills, K. A., Mathews, K. D., Scherpbier-Heddema, T., Buetow, K. H., Baldini, A., Ballabio, A., Borsani, G. Genetic and physical mapping of a voltage-dependent chloride channel gene to human 4q32 and to mouse 8. Genomics 36: 374-376, 1996.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

170 kDa

Applications Notes

6

Isotype

IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.