Anti-Collagen Type II/COL2A1 Antibody Picoband®

About this product

Boster Bio Anti-Collagen Type II/COL2A1 Antibody Picoband® catalog # A00517-2. Tested in WB, IHC, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00517-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital), also known as COL2A1, is a human gene that provides instructions for the production of the pro-alpha1 (II) chain of type II collagen. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.

Gene Name

COL2A1

Gene ID

1280

UniProt

P02458

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human Collagen Type II/COL2A1 recombinant protein (Position: G1217-A1241) . Human COL2A1 shares 92% and 96% amino acid (aa) sequence identity with mouse and rat COL2A1, respectively.

Clonality

Polyclonal

Applications

WB, IHC, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Ahmad, N. N., Ala-Kokko, L., Knowlton, R. G., Jimenez, S. A., Weaver, E. J., Maguire, J. I., Tasman, W., Prockop, D. J. Stop codon in the procollagen II gene (COL2A1) in a family with the Stickler syndrome (arthro-ophthalmopathy) . Proc. Nat. Acad. Sci. 88: 6624-6627, 1991. 2. Ahmad, N. N., Ala-Kokko, L., Knowlton, R. G., Weaver, E. J., Maguire, J. I., Tasman, W., Prockop, D. J. A stop codon in the gene for type II procollagen (COL2A1) causes one variant of arthro-ophthalmopathy (the Stickler syndrome) . (Abstract) Am. J. Hum. Genet. 47 (suppl.) : A206 only, 1990. 3. Ahmad, N. N., Dimascio, J., Knowlton, R. G., Tasman, W. S. Stickler syndrome: a mutation in the nonhelical 3-prime end of type II procollagen gene. Arch. Ophthal. 113: 1454-1457, 1995.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

142 kDa

Observed Molecular Weight

150-180 kDa

Isotype

IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.