Anti-NCLN Antibody Picoband®

About this product

Boster Bio Anti-NCLN Antibody Picoband® catalog # A12284-2. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A12284-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nicalin is a protein that in humans is encoded by the NCLN gene. Enables ribosome binding activity. Involved in protein stabilization; regulation of protein complex stability; and regulation of protein-containing complex assembly. Located in endoplasmic reticulum membrane. Part of protein-containing complex.

Gene Name

NCLN

Gene ID

56926

UniProt

Q969V3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human NCLN recombinant protein (Position: Q59-Q563) . Human NCLN shares 92.9% and 92.5% amino acid (aa) sequence identity with mouse and rat NCLN, respectively.

Clonality

Polyclonal

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Dettmer, U., Kuhn, P.-H., Abou-Ajram, C., Lichtenthaler, S. F., Kruger, M., Kremmer, E., Haass, C., Haffner, C. Transmembrane protein 147 (TMEM147) is a novel component of the nicalin-NOMO protein complex. J. Biol. Chem. 285: 26174-26181, 2010. 2. Haffner, C., Frauli, M., Topp, S., Irmler, M., Hofmann, K., Regula, J. T., Bally-Cuif, L., Haass, C. Nicalin and its binding partner Nomo are novel Nodal signaling antagonists. EMBO J. 23: 3041-3050, 2004. 3. Stumpf, A. M. Personal Communication. Baltimore, Md. 08/17/2021.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

63 kDa

Observed Molecular Weight

63 kDa

Applications Notes

6

Isotype

IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.