Anti-HEB/TCF12 Antibody Picoband®

About this product

Boster Bio Anti-HEB/TCF12 Antibody Picoband® catalog # A02592-3. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02592-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transcription factor 12 is a protein that in humans is encoded by the TCF12 gene. The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Synonyms

TCF12; BHLHB20; HEB; HTF4; Transcription factor 12; TCF-12; Class B basic helix-loop-helix protein 20; bHLHb20; DNA-binding protein HTF4; E-box-binding protein; Transcription factor HTF-4

Gene Name

TCF12

Gene ID

6938

UniProt

Q99081

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human HEB/TCF12 recombinant protein (Position: S67-G300) . Human TCF12 shares 96.6% amino acid (aa) sequence identity with both mouse and rat TCF12.

Clonality

Polyclonal

Applications

WB, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Davis, E. E., Balasubramanian, R., Kupchinsky, Z. A., Keefe, D. L., Jr., Plummer, L., Khan, K., Meczekalski, B., Heath, K. E., Lopez-Gonzalez, V., Ballesta-Martinez, M. J., Margabanthu, G., Price, S., and 12 others. TCF12 haploinsufficiency causes autosomal dominant Kallmann syndrome and reveals network-level interactions between causal loci. Hum. Molec. Genet. 29: 2435-2450, 2020. 2. Doyle, K., Zhang, Y., Baer, R., Bina, M. Distinguishable patterns of protein-DNA interactions involving complexes of basic helix-loop-helix proteins. J. Biol. Chem. 269: 12099-12105, 1994. 3. Gan, T.-I., Rowen, L., Nesbitt, R., Roe, B. A., Wu, H., Hu, P., Yao, Z., Kim, U.-J., O'Sickey, T., Bina, M. Genomic organization of human TCF12 gene and spliced mRNA variants producing isoforms of transcription factor HTF4. Cytogenet. Genome Res. 98: 245-248, 2002.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

73 kDa

Observed Molecular Weight

85 kDa

Applications Notes

6

Gene Name Synonym

Transcription factor 12

Protein Name

Transcription factor 12

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.