Anti-PHGDH Antibody Picoband®

About this product

Boster Bio Anti-PHGDH Antibody Picoband® catalog # A03355-1. Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03355-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

Synonyms

PHGDH; PGDH3; D-3-phosphoglycerate dehydrogenase; 3-PGDH; EC 1.1.1.95; 2-oxoglutarate reductase; EC 1.1.1.399; Malate dehydrogenase; EC 1.1.1.37

Gene Name

PHGDH

Gene ID

26227

UniProt

O43175

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PHGDH recombinant protein (Position: L15-F533) . Human PHGDH shares 94.4% and 94.2% amino acid (aa) sequence identity with mouse and rat PHGDH, respectively.

Clonality

Polyclonal

Applications

ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Acuna-Hidalgo, R., Schanze, D., Kariminejad, A., Nordgren, A., Kariminejad, M. H., Conner, P., Grigelioniene, G., Nilsson, D., Nordenskjold, M., Wedell, A., Freyer, C., Wredenberg, A., and 18 others. Neu-Laxova syndrome is a heterogeneous metabolic disorder caused by defects in enzymes of the L-serine biosynthesis pathway. Am. J. Hum. Genet. 95: 285-293, 2014. 2. Baek, J. Y., Jun, D. Y., Taub, D., Kim, Y. H. Assignment of human 3-phosphoglycerate dehydrogenase (PHGDH) to human chromosome band 1p12 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 89: 6-7, 2000. 3. Benke, P. J., Hidalgo, R. J., Braffman, B. H., Jans, J., van Gassen, K. L. I., Sunbul, R., El-Hattab, A. W. Infantile serine biosynthesis defect due to phosphoglycerate dehydrogenase deficiency: variability in phenotype and treatment response, novel mutations, and diagnostic challenges. J. Child Neurol. 32: 543-549, 2017.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

57 kDa

Observed Molecular Weight

57 kDa

Applications Notes

6

Gene Name Synonym

Phosphoglycerate dehydrogenase

Protein Name

D-3-phosphoglycerate dehydrogenase

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.