Anti-CYP7B1 Antibody Picoband®

About this product

Boster Bio Anti-CYP7B1 Antibody Picoband® catalog # A03545-3. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03545-3-carrier-free

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder.

Synonyms

CYP7B1; Cytochrome P450 7B1; 24-hydroxycholesterol 7-alpha-hydroxylase; EC 1.14.14.26; 25/26-hydroxycholesterol 7-alpha-hydroxylase; EC 1.14.14.29; 3-hydroxysteroid 7-alpha hydroxylase; Oxysterol 7-alpha-hydroxylase

Gene Name

CYP7B1

Gene ID

9420

UniProt

O75881

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human CYP7B1 recombinant protein (Position: Q127-D391) . Human CYP7B1 shares 62.3% and 63% amino acid (aa) sequence identity with mouse and rat CYP7B1, respectively.

Clonality

Polyclonal

Applications

WB, IHC, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Arnoldi, A., Crimella, C., Tenderini, E., Martinuzzi, A., D'Angelo, M. G., Musumeci, O., Toscano, A., Scarlato, M., Fantin, M., Bresolin, N., Bassi, M. T. Clinical phenotype variability in patients with hereditary spastic paraplegia type 5 associated with CYP7B1 mutations. Clin. Genet. 81: 150-157, 2012. 2. Biancheri, R., Ciccolella, M., Rossi, A., Tessa, A., Cassandrini, D., Minetti, C., Santorelli, F. M. White matter lesions in spastic paraplegia with mutations in SPG5/CYP7B1. Neuromusc. Disord. 19: 62-65, 2009. 3. Biddinger, S. B., Haas, J. T., Yu, B. B., Bezy, O., Jing, E., Zhang, W., Unterman, T. G., Carey, M. C., Kahn, C. R. Hepatic insulin resistance directly promotes formation of cholesterol gallstones. Nature Med. 14: 778-782, 2008.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

58 kDa

Observed Molecular Weight

58 kDa

Applications Notes

6

Gene Name Synonym

Cytochrome P450 family 7 subfamily B member 1

Protein Name

Cytochrome P450 7B1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.