Anti-NSMAF Antibody Picoband®

About this product

Boster Bio Anti-NSMAF Antibody Picoband® catalog # A09560. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A09560

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.

Synonyms

NSMAF; FAN; Protein FAN; Factor associated with neutral sphingomyelinase activation; Factor associated with N-SMase activation

Gene Name

NSMAF

Gene ID

8439

UniProt

Q92636

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human NSMAF recombinant protein (Position: K51-L868) . Human NSMAF shares 92.3% amino acid (aa) sequence identity with mouse NSMAF.

Clonality

Polyclonal

Applications

WB, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Adam-Klages, S., Adam, D., Wiegmann, K., Struve, S., Kolanus, W., Schneider-Mergener, J., Kronke, M. FAN, a novel WD-repeat protein, couples the p55 TNF-receptor to neutral sphingomyelinase. Cell 86: 937-947, 1996. 2. Montfort, A., de Badts, B., Douin-Echinard, V., Martin, P. G. P., Iacovoni, J., Nevoit, C., Therville, N., Garcia, V., Bertrand, M.-A., Bessieres, M.-H., Trombe, M.-C., Levade, T., Benoist, H., Segui, B. FAN stimulates TNF-alpha-induced gene expression, leukocyte recruitment, and humoral response. J. Immun. 183: 5369-5378, 2009. 3. Segui, B., Cuvillier, O., Adam-Klages, S., Garcia, V., Malagarie-Cazenave, S., Leveque, S., Caspar-Bauguil, S., Coudert, J., Salvayre, R., Kronke, M., Levade, T. Involvement of FAN in TNF-induced apoptosis. J. Clin. Invest. 108: 143-151, 2001.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

104 kDa

Observed Molecular Weight

104 kDa

Applications Notes

6

Gene Name Synonym

Neutral sphingomyelinase activation associated factor

Protein Name

Protein FAN

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.