Anti-SPRED1 Antibody Picoband®

About this product

Boster Bio Anti-SPRED1 Antibody Picoband® catalog # A03613-1. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03613-1-carrier-free

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS) .

Synonyms

SPRED1; Sprouty-related, EVH1 domain-containing protein 1; Spred-1; hSpred1

Gene Name

SPRED1

Gene ID

161742

UniProt

Q7Z699

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SPRED1 recombinant protein (Position: F45-K322) . Human SPRED1 shares 89.9% amino acid (aa) sequence identity with mouse SPRED1.

Clonality

Polyclonal

Applications

WB, ICC/IF, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Ablain, J., Xu, M., Rothschild, H., Jordan, R. C., Mito, J. K., Daniels, B. H., Bell, C. F., Joseph, N. M., Wu, H., Bastian, B. C., Zon, L. I., Yeh, I. Human tumor genomics and zebrafish modeling identify SPRED1 loss as a driver of mucosal melanoma. Science 362: 1055-1060, 2018. 2. Brems, H., Chmara, M., Sahbatou, M., Denayer, E., Taniguchi, K. Kato, R., Somers, R., Messiaen, L., De Schepper, S., Fryns, J.-P., Cools, J., Marynen, P., Thomas, G., Yoshimura, A., Legius, E. Germline loss-of-function mutations in SPRED1 cause a neurofibromatosis 1-like phenotype. (Letter) Nature Genet. 39: 1120-1126, 2007. 3. Brems, H., Pasmant, E., Van Minkelen, R., Wimmer, K., Upadhyaya, M., Legius, E., Messiaen, L. Review and update of SPRED1 mutations causing Legius syndrome. Hum. Mutat. 33: 1538-1546, 2012.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

50 kDa

Observed Molecular Weight

50 kDa

Applications Notes

6

Gene Name Synonym

Sprouty related EVH1 domain containing 1

Protein Name

Sprouty-related, EVH1 domain-containing protein 1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.