Anti-NEK7 Antibody Picoband®

About this product

Boster Bio Anti-NEK7 Antibody Picoband® catalog # A04996-1. Tested in WB, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A04996-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.

Synonyms

NEK7; Serine/threonine-protein kinase Nek7; EC 2.7.11.1; Never in mitosis A-related kinase 7; NimA-related protein kinase 7

Gene Name

NEK7

Gene ID

140609

UniProt

Q8TDX7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human NEK7 recombinant protein (Position: M1-S302) . Human NEK7 shares 97.7% and 98% amino acid (aa) sequence identity with mouse and rat NEK7, respectively.

Clonality

Polyclonal

Applications

WB, ICC/IF, IP, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. He, Y., Zeng, M. Y., Yang, D., Motro, B., Nunez, G. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature 530: 354-357, 2016. 2. Kimura, M., Okano, Y. Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3. Cytogenet. Cell Genet. 94: 33-38, 2001. 3. Sharif, H., Wang, L., Wang, W. L., Magupalli, V. G., Andreeva, L., Qiao, Q., Hauenstein, A. V., Wu, Z., Nunez, G., Mao, Y., Wu, H. Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome. Nature 570: 338-343, 2019.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

35 kDa

Observed Molecular Weight

35 kDa

Applications Notes

6

Gene Name Synonym

NIMA related kinase 7

Protein Name

Serine/threonine-protein kinase Nek7

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.