Anti-PUF60 Antibody Picoband®

About this product

Boster Bio Anti-PUF60 Antibody Picoband® catalog # A05744-2. Tested in WB, IHC, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05744-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Synonyms

PUF60; FIR; ROBPI; SIAHBP1; Poly (U-binding-splicing factor PUF60; 60 kDa poly (U-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1

Gene Name

PUF60

Gene ID

22827

UniProt

Q9UHX1

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PUF60 recombinant protein (Position: Q48-A559) . Human PUF60 shares 98.8% amino acid (aa) sequence identity with both mouse and rat PUF60.

Clonality

Polyclonal

Applications

WB, IHC, IP, Flow Cytometry, ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

References & Citations

1. Dauber, A., Golzio, C., Guenot, C., Jodelka, F. M., Kibaek, M., Kjaergaard, S., Leheup, B., Martinet, D., Nowaczyk, M. J. M., Rosenfeld, J. A., Zeesman, S., Zunich, J., Beckmann, J. S., Hirschhorn, J. N., Hastings, M. L., Jacquemont, S., Katsanis, N. SCRIB and PUF60 are primary drivers of the multisystemic phenotypes of the 8q24.3 copy-number variant. Am. J. Hum. Genet. 93: 798-811, 2013. Note: Erratum: Am. J. Hum. Genet. 93: 994 only, 2013. 2. El Chehadeh, S., Kerstjens-Frederikse, W. S., Thevenon, J., Kuentz, P., Bruel, A.-L., Thauvin-Robinet, C., Bensignor, C., Dollfus, H., Laugel, V., Riviere, J.-B., Duffourd, Y., Bonnet, C., and 19 others. Dominant variants in the splicing factor PUF60 cause a recognizable syndrome with intellectual disability, heart defects and short stature. Europ. J. Hum. Genet. 25: 43-51, 2017. 3. Graziano, C., Gusson, E., Severi, G., Isidori, F., Wischmeijer, A., Brugnara, M., Seri, M., Rossi, C. A de novo PUF60 mutation in a child with a syndromic form of coloboma and persistent fetal vasculature. Ophthal. Genet. 38: 590-592, 2017.

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

60 kDa

Observed Molecular Weight

60 kDa

Applications Notes

6

Gene Name Synonym

Poly (U) binding splicing factor 60

Protein Name

Poly (U) -binding-splicing factor PUF60

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.