- Catalog number
- MA1000
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
- Synonyms
- Actin, alpha skeletal muscle; Alpha-actin-1; ACTA1; ACTA
- Gene Name
- Actin
- Gene ID
- Human (58), Mouse (11459), Rat (29437), Human (70), Mouse (11464), Rat (29275), Human (59), Mouse (11475), Rat (81633), Human (60), Mouse (11461), Rat (81822), Human (71), Mouse (11465), Rat (287876), Human (72), Mouse (11468), Rat (25365)
- UniProt
- P68133
- Host
- Mouse
- Reactivity
- Chicken, Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.
- Clonality
- Monoclonal
- Clone
- Clone: AC-40
- Tissue Specificity
- Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
- Applications
- IHC, WB
- Field of Research
- Actin, Actin Assembly, Actin Binding Proteins, Actin, etc., Apoptosis, Cancer, Cell Death, Cytoskeleton, Cytoskeleton/ECM, Invasion/Microenvironment, Microfilaments, Receptors, Signal Transduction, Subcellular Markers, Tags & Cell Markers
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
- References & Citations
- 1. Gunning, P., Ponte, P., Okayama, H., Engel, J., Blau, H. and Kedes, L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Mol. Cell. Biol.1983; 3 (5), 787-795. 2. Goebel, H.H., Brockmann, K., Bonnemann, C.G., Warlo, I.A., Hanefeld, F., Labeit, S., Durling, H.J. and Laing, N.G.Actin-related myopathy without any missense mutation in the ACTA1 Gene. J. Child Neurol.2004; 19 (2), 149-153. 3. Laing, N.G., Clarke, N.F., Dye, D.E., Liyanage, K., Walker, K.R., Kobayashi, Y., Shimakawa, S., Hagiwara, T., Ouvrier, R., Sparrow, J.C., Nishino, I., North, K.N. and Nonaka, I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004; 56 (5), 689-694.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 42051 MW
- Observed Molecular Weight
- 43 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Actin, alpha skeletal muscle
- Subcellular Location
- Cytoplasm, cytoskeleton.
- Protein Name
- Actin, alpha skeletal muscle|Actin, alpha cardiac muscle 1|Actin, aortic smooth muscle|Actin, cytoplasmic 1|Actin, cytoplasmic 2|Actin, gamma-enteric smooth muscle
- Isotype
- Mouse IgG2a
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
