Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody

About this product

Boster Bio Anti-AFP/Alpha 1 Fetoprotein Monoclonal Antibody catalog # MA1001. Tested in IHC applications. This antibody reacts with Human.

Specifications

Catalog number

MA1001

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alpha-fetoprotein (AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk (1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility.

Synonyms

Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; Afp

Gene Name

AFP

Gene ID

Human (174), Mouse (11576), Rat (24177)

UniProt

P02773

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human alpha-fetoprotein.

Clonality

Monoclonal

Clone

Clone: C3

Tissue Specificity

Plasma. .

Applications

IHC

Field of Research

Serum Proteins, Tumor Antigens, Tumor Biomarkers

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Binds estrogens, fatty acids and metals.

References & Citations

1. D'Eustachio, P.; Ingram, R. S.; Tilghman, S. M.; Ruddle, F. H. : Murine alpha-fetoprotein and albumin: two evolutionarily linked proteins encoded on the same mouse chromosome. Somat. Cell Genet. 7: 289-294, 1981. 2. Beattie, W. G.; Dugaiczyk, A. : Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA. Gene 20: 415-422, 1982. 3. Gabant, P.; Forrester, L.; Nichols, J.; Van Reeth, T.; De Mees, C.; Pajack, B.; Watt, A.; Smitz, J.; Alexandre, H.; Szpirer, C.; Szpirer, J. : Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility. Proc. Nat. Acad. Sci. 99: 12865-12870, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68386 MW

Observed Molecular Weight

43 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Alpha-fetoprotein

Subcellular Location

Isoform 1: Secreted.

Sequence Similarities

Belongs to the ALB/AFP/VDB family.

Protein Name

Alpha-fetoprotein

Isotype

Mouse IgG2a

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.