Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)

About this product

Boster Bio Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100) catalog # MA1004. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

MA1004

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Synonyms

Apoptosis regulator Bcl-2; Bcl2; Bcl-2

Gene Name

BCL2

Gene ID

Human (596), Mouse (12043), Rat (24224)

UniProt

P49950

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.

Clonality

Monoclonal

Clone

Clone: Bcl-2-100

Tissue Specificity

Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, and in ependymal cells. In prenatal brain, expression is higher and localized in the neuroepithelium and in the cortical plate.

Applications

IHC, ICC, WB

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Cancer Metabolism, Cell Biology, Cell Death, Hypoxia, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oncoproteins, Oncoproteins/Suppressors, Pathways and Processes, Response To Hypoxia, Signal Transduction, Tumor Biomarkers

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) . May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (By similarity) . .

References & Citations

1. Ngan, B.-Y.; Chen-Levy, Z.; Weiss, L. M.; Warnke, R. A.; Cleary, M. L. : Expression in non-Hodgkin's lymphoma of the BCL-2 protein associated with the t (14;18) chromosomal translocation. New Eng. J. Med. 318: 1638-1644, 1988. 2. Hockenbery, D.; Nunez, G.; Milliman, C.; Schreiber, R. D.; Korsmeyer, S. J. : Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26622 MW

Observed Molecular Weight

26 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Apoptosis regulator Bcl-2

Subcellular Location

Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Sequence Similarities

Belongs to the Bcl-2 family.

Protein Name

Apoptosis regulator Bcl-2

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.