Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD)

About this product

Boster Bio Anti-Caldesmon (Smooth) Cald1 Antibody (Monoclonal, hHCD) catalog # MA1009. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit.

Specifications

Catalog number

MA1009

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon (h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon (l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al. (1992) demonstrated that the human CDM gene is composed of 14 exons.

Synonyms

Non-muscle caldesmon; CDM; L-caldesmon; Cald1

Gene Name

CALD1

Gene ID

Human (800), Mouse (109624), Rat (25687)

UniProt

Q62736

Host

Mouse

Reactivity

Human, Mouse, Rabbit

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human uterus smooth muscle extract.

Clonality

Monoclonal

Clone

Clone: hHCD

Tissue Specificity

High-molecular-weight caldesmon (h-caldesmon) is predominantly expressed in smooth muscles, whereas low- molecular-weight caldesmon (l-caldesmon) is widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Applications

IHC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Ascites

Concentration

Add 1ml of PBS buffer will yield a concentration of 100 ug/ml

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100 ug/ml

Function

Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments) . Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration. .

References & Citations

1. Humphrey, M. B.; Herrera-Sosa, H.; Gonzalez, G.; Lee, R.; Bryan, J. : Cloning of cDNAs encoding human caldesmons. Gene 112: 197-204, 1992. 2. Hayashi, K.; Yano, H.; Hashida, T.; Takeuchi, R.; Takeda, O.; Asada, K.; Takahashi, E.; Kato, I.; Sobue, K. : Genomic structure of the human caldesmon gene. Proc. Nat. Acad. Sci. 89: 12122-12126, 1992.

Storage Conditions

At -20°C for one year and at 4°C for one month from date of receipt. Avoid repeated freezing and thawing.

Calculated Molecular Weight

60584 MW

Observed Molecular Weight

61 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Non-muscle caldesmon

Subcellular Location

Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) . .

Sequence Similarities

Belongs to the calcineurin regulatory subunit family.

Protein Name

Caldesmon

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.