Anti-CD3 CD3E Antibody (Monoclonal, CA-3)

About this product

Boster Bio Anti-CD3 CD3E Antibody (Monoclonal, CA-3) catalog # MA1015. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.

Specifications

Catalog number

MA1015

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3) . Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.

Synonyms

CD3 antigen, epsilon polypeptide (Predicted) ; Protein Cd3e ; Cd3e ; Cd3e_predicted ; rCG_58471

Gene Name

CD3E

Gene ID

Human (916), Mouse (12501), Rat (315609)

UniProt

D4A5M2

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human thymocytes followed by Sezary T cells.

Clonality

Monoclonal

Clone

Clone: CA-3

Tissue Specificity

Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .

Applications

Flow Cytometry, IHC, ICC

Field of Research

Adaptive Immunity, Hematopoietic Progenitors, Immunology, Stem Cells, T Cells

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

CD3E: The CD3 complex mediates signal transduction.

References & Citations

1. Krissansen, G. W.; Owen, M. J.; Verbi, W.; Crumpton, M. J. : Primary structure of the T3 gamma subunit of the T3/T cell antigen receptor complex deduced from cDNA sequences: evolution of the T3 gamma and delta subunits. EMBO J. 5: 1799-1808, 1986. 2. Tunnacliffe, A.; Buluwela, L.; Rabbitts, T. H. : Physical linkage of three CD3 genes on human chromosome 11. EMBO J. 6: 2953-2957, 1987.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21312 MW

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

CD3e molecule, epsilon (CD3-TCR complex)

Subcellular Location

CD3E: Membrane; Single-pass type I membraneprotein.|Cd3e: Cell membrane

Sequence Similarities

Contains 1 Ig-like (immunoglobulin-like) domain.

Protein Name

T-cell surface glycoprotein CD3 epsilon chain

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.