- Catalog number
- MA1016
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD stands for cluster of differentiation; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T, gp55]. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
- Synonyms
- T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD4; Cd4
- Gene Name
- CD4
- Gene ID
- Human (920), Mouse (12504), Rat (24932)
- UniProt
- P05540
- Host
- Mouse
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.
- Clonality
- Monoclonal
- Clone
- Clone: CA-4
- Tissue Specificity
- Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .
- Applications
- Flow Cytometry, IHC, ICC
- Field of Research
- Adaptive Immunity, Antiviral Signaling, Hematopoietic Progenitors, Immune System Diseases, Immunology, Lymphoid, Myeloid, Stem Cells, T Cells, T Lymphocytic Lineage
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.
- References & Citations
- 1. Committee on Human Leukocyte Differentiation Antigens, IUIS WHO Nomenclature Subcommittee : Proposed nomenclature for human leukocyte differentiation antigens. Bull. WHO 5: 809-811, 1984. Note: Alternate: Immunology Today 5: 280 only, 1984. 2. Grakoui, A.; Bromley, S. K.; Sumen, C.; Davis, M. M.; Shaw, A. S.; Allen, P. M.; Dustin, M. L. : The immunological synapse: a molecular machine controlling T cell activation. Science 285: 221-227, 1999.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 51438 MW
- Observed Molecular Weight
- 25 kDa
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- 6
- Gene Name Synonym
- T-cell surface glycoprotein CD4
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
- Protein Name
- T-cell surface glycoprotein CD4
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
