Anti-CDC25C Antibody (Monoclonal, DCS-193)

About this product

Boster Bio Anti-CDC25C Antibody (Monoclonal, DCS-193) catalog # MA1018. Tested in WB applications. This antibody reacts with Human.

Specifications

Catalog number

MA1018

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cell division sycle (CDC25) gene product is a protein-tyrosine phosphatase, activates a partially purified p34 (cdc2) /cyclin B complex. The cdc25 protein also shares homology with a protein phosphatase with activity against both tyrosine and serine (and thus probably threonine) phosphate residues. CDC25C is expressed predominantly in G2 phase in healer cells. The human gene encodes a protein with a predicted molecular mass of 53,000 daltons whose C-terminal domain shares about 37% sequence identity with the fission yeast cdc25 mitotic inducer. CDC25C gene is mapped to 5q31.

Synonyms

Protein Cdc25c ; Cdc25c

Gene Name

CDC25C

Gene ID

Human (995), Mouse (12532), Rat (307511)

UniProt

D4ADT2

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Recombinant human Cdc25c.

Clonality

Monoclonal

Clone

Clone: DCS-193

Applications

WB

Field of Research

Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

CDC25C: Functions as a dosage-dependent inducer in mitoticcontrol. Tyrosine protein phosphatase required for progression ofthe cell cycle. When phosphorylated, highly effective inactivating G2 cells into prophase. ly dephosphorylates CDK1and activates its kinase activity.

References & Citations

1. Sartor, H.; Ehlert, F.; Grzeschik, K.-H.; Muller, R.; Adolph, S. : Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively. Genomics 13: 911-912, 1992. 2. Taviaux, S. A.; Demaille, J. G. : Localization of human cell cycle regulatory genes CDC25C to 5q31 and WEE1 to 11p15.3-11p.15.1 by fluorescence in situ hybridization. Genomics 15: 194-196, 1993.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50896 MW

Observed Molecular Weight

25 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cell division cycle 25 homolog C (S. pombe)

Subcellular Location

CDC25C: Nucleus |Cdc25c: Nucleus.

Sequence Similarities

Belongs to the MPI phosphatase family.

Protein Name

M-phase inducer phosphatase 3

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.