- Catalog number
- MA1019
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- In yeasts, Cdc6 (Saccharomyces cerevisiae) and Cdc18 (Schizosaccharomyces pombe) associate with the origin recognition complex (ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al. (1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.
- Synonyms
- Cell division control protein 6 homolog; CDC6-related protein; p62 (cdc6) ; Cdc6
- Gene Name
- CDC6
- Gene ID
- Human (990), Mouse (23834), Rat (360621)
- UniProt
- O89033
- Host
- Mouse
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Recombinant human Cdc6.
- Clonality
- Monoclonal
- Clone
- Clone: DCS-180
- Applications
- WB
- Field of Research
- Cell Biology, Cell Cycle, Cell Division, DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.
- References & Citations
- 1. Gonzalez, S.; Klatt, P.; Delgado, S.; Conde, E.; Lopez-Rios, F.; Sanchez-Cespedes, M.; Mendez, J.; Antequera, F.; Serrano, M. : Oncogenic activity of Cdc6 through repression of the INK4/ARF locus. Nature 440: 702-706, 2006. 2. Yan, Z.; DeGregori, J.; Shohet, R.; Leone, G.; Stillman, B.; Nevins, J. R.; Williams, R. S. : Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells. Proc. Nat. Acad. Sci. 95: 3603-3608, 1998.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 62614 MW
- Observed Molecular Weight
- 25 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Cell division control protein 6 homolog
- Subcellular Location
- Nucleus . Cytoplasm . The protein is nuclear in G1 and cytoplasmic in S-phase cells. .
- Sequence Similarities
- Belongs to the CDC6/cdc18 family.
- Protein Name
- Cell division control protein 6 homolog
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
