Anti-CDK4 Antibody (Monoclonal, DCS-31)

About this product

Boster Bio Anti-CDK4 Antibody (Monoclonal, DCS-31) catalog # MA1020. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

MA1020

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.

Gene Name

CDK4

Gene ID

Human (1019), Mouse (12567), Rat (94201)

UniProt

P11802

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Recombinant human Cdk4 protein.

Clonality

Monoclonal

Clone

Clone: DCS-31

Tissue Specificity

Brain.

Applications

ICC, WB

Field of Research

Neuroscience, Neurotransmission, Receptors / Channels

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. .

References & Citations

1. Demetrick, D. J.; Zhang, H.; Beach, D. H. : Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes. Cytogenet. Cell Genet. 66: 72-74, 1994. 2. Modiano, J. F.; Mayor, J.; Ball, C.; Fuentes, M. K.; Linthicum, D. S. : CDK4 expression and activity are required for cytokine responsiveness in T cells. J. Immun. 165: 6693-6702, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

44225 MW

Observed Molecular Weight

34 kDa

Specificity

No cross reactivity with other proteins.

Subcellular Location

Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes) . DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells.

Sequence Similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Protein Name

Cyclin-dependent kinase 4

Isotype

Mouse IgG2a

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.