- Catalog number
- MA1021
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The cyclin-dependent protein kinases (CDKs) regulate major cell cycle transitions in eukaryotic cells. Cyclin-dependent kinase-6 (CDK6) is the earliest inducible member of the CDK family in human T lymphocytes, involved in growth factor stimulation and cell cycle progression. Germline mutations in CDK6 do not make a significant contribution to melanoma predisposition. CDK6 gene is mapped at 7q21-q22.
- Synonyms
- Cyclin-dependent kinase 6 ; Protein Cdk6 ; Cdk6 ; rCG_41083
- Gene Name
- CDK6
- Gene ID
- Human (1021), Mouse (12571), Rat (114483)
- UniProt
- F1MA87
- Host
- Mouse
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Recombinant human Cdk6 protein.
- Clonality
- Monoclonal
- Clone
- Clone: DCS-90
- Tissue Specificity
- CDK6: Expressed ubiquitously. Accumulates insquamous cell carcinomas, proliferating hematopoietic progenitorcells, beta-cells of pancreatic islets of Langerhans, andneuroblastomas. Reduced levels in differentiating cells.
- Applications
- ICC, WB
- Field of Research
- Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- CDK6: Serine/threonine-protein kinase involved in the controlof the cell cycle and differentiation; promotes G1/S transition.Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclinsduring interphase at G1 to form a pRB/RB1 kinase and controls theentrance into the cell cycle. Involved in initiation andmaintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively celldifferentiation, but is required for the proliferation of specificcell types (e.g. erythroid and hematopoietic cells) . Essential forcell proliferation within the dentate gyrus of the hippocampus andthe subventricular zone of the lateral ventricles. Required duringthymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least inastrocytes, changes in patterns of gene expression, changes in theactin cytoskeleton including loss of stress fibers, and enhancedmotility during cell differentiation. Prevents myeloiddifferentiation by interfering with RUNX1 and reducing itstranscription transactivation activity, but promotes proliferationof normal myeloid progenitors. Delays senescence. Promotes theproliferation of beta-cells in pancreatic islets of Langerhans.May play a role in the centrosome organization during the cellcycle phases (PubMed:23918663) .
- References & Citations
- 1. Chilosi, M.; Doglioni, C.; Yan, Z.; Lestani, M.; Menestrina, F.; Sorio, C.; Benedetti, A.; Vinante, F.; Pizzolo, G.; Inghirami, G. : Differential expression of cyclin-dependent kinase 6 in cortical thymocytes and T-cell lymphoblastic lymphoma/leukemia. Am. J. Path. 152: 209-217, 1998. 2. Shennan, M. G.; Badin, A.-C.; Walsh, S.; Summers, A.; From, L.; McKenzie, M.; Goldstein, A. M.; Tucker, M. A.; Hogg, D.; Lassam, N. : Lack of germline CDK6 mutations in familial melanoma. Oncogene 19: 1849-1852, 2000. 3. Lien, H.-C.; Lin, C.-W.; Huang, P.-H.; Chang, M.-L.; Hsu, S.-M. : Expression of cyclin-dependent kinase 6 (cdk6) and frequent loss of CD44 in nasal-nasopharyngeal NK/T-cell lymphomas: comparison with CD56-negative peripheral T-cell lymphomas. Lab. Invest. 80: 893-900, 2000.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 36952 MW
- Observed Molecular Weight
- 37 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Cyclin-dependent kinase 6
- Subcellular Location
- CDK6: Cytoplasm. Nucleus. Cell projection, ruffle.Cytoplasm, cytoskeleton, microtubule organizing center, centrosome|Cdk6: Cytoplasm
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
- Protein Name
- Cyclin-dependent kinase 6
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
