Anti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68)

About this product

Boster Bio Anti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68) catalog # MA1024. Tested in IHC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

MA1024

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.

Synonyms

Keratin, type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Type-II keratin Kb7; Krt7 ; Kb7 , Krt2-7

Gene Name

KRT7

Gene ID

Human (3855), Mouse (110310), Rat (300242)

UniProt

Q6IG12

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Cytoskeletal preparation of the RT4 human bladder carcinoma cell line.

Clonality

Monoclonal

Clone

Clone: LDS-68

Tissue Specificity

KRT7: Expressed in cultured epidermal, bronchial andmesothelial cells but absent in colon, ectocervix and liver.Observed throughout the glandular cells in the junction betweenstomach and esophagus but is absent in the esophagus.

Applications

IHC, WB

Field of Research

Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Signal Transduction

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. .

References & Citations

1. Rosenberg, M.; Fuchs, E.; Le Beau, M. M.; Eddy, R. L.; Shows, T. B. : Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12. Cytogenet. Cell Genet. 57: 33-38, 1991. 2. Smith, F. J. D.; Porter, R. M.; Corden, L. D.; Lunny, D. P.; Lane, E. B.; McLean, W. H. I. : Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse. Biochem. Biophys. Res. Commun. 297: 818-827, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50709 MW

Observed Molecular Weight

37 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Keratin, type II cytoskeletal 7

Subcellular Location

KRT7: Cytoplasm

Sequence Similarities

Belongs to the intermediate filament family.

Protein Name

Keratin, type II cytoskeletal 7

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.