- Catalog number
- MA1029
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.
- Synonyms
- Collagen alpha-1 (III) chain; Col3a1
- Gene Name
- COL3A1
- Gene ID
- Human (1281), Mouse (12825), Rat (84032)
- UniProt
- P13941
- Host
- Mouse
- Reactivity
- Human, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Human collagen type III.
- Clonality
- Monoclonal
- Clone
- Clone: FH-7A
- Tissue Specificity
- Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .
- Applications
- IHC, WB
- Field of Research
- Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 (By similarity) . .
- References & Citations
- 1. Cutting, G. R.; McGinniss, M. J.; Kasch, I. M.; Tsipouras, P.; Antonarakis, S. E. : Physical mapping by PFGE localizes the COL3A1 and COL5A2 genes to a 35 kb region on chromosome 2. (Abstract) Clin. Res. 38: 266A, 1990. 2. Emanuel, B. S.; Cannizzaro, L. A.; Seyer, J. M.; Myers, J. C. : Human alpha-1 (III) and alpha-2 (V) procollagen genes are located on the long arm of chromosome 2. Proc. Nat. Acad. Sci. 82: 3385-3389, 1985. 3. Liu, X.; Wu, H.; Byrne, M.; Krane, S.; Jaenisch, R. : Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development. Proc. Nat. Acad. Sci. 94: 1852-1856, 1997.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 138936 MW
- Observed Molecular Weight
- 49 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Collagen alpha-1 (III) chain
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Belongs to the fibrillar collagen family.
- Protein Name
- Collagen alpha-1 (III) chain
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
