- Catalog number
- MA1034
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Inaba et al. (1992) used murine cDNA clones for 3 cyclin D genes that are normally expressed during the G1 phase of the cell cycle to clone the cognate human genes. D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. cyclin D2 (CCND2) gene ia assigned to 12p13.
- Synonyms
- G1/S-specific cyclin-D2; Vin-1 proto-oncogene; Ccnd2; Vin-1
- Gene Name
- CCND2
- Gene ID
- Human (894), Mouse (12444), Rat (64033)
- UniProt
- Q04827
- Host
- Mouse
- Reactivity
- Human, Mouse
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Recombinant human cyclin D2 protein.
- Clonality
- Monoclonal
- Clone
- Clone: DCS-3
- Applications
- ICC, WB
- Field of Research
- Cancer, Cell Biology, Cell Cycle, Epigenetics and Nuclear Signaling
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity) . .
- References & Citations
- 1. Kozar, K.; Ciemerych, M. A.; Rebel, V. I.; Shigematsu, H.; Zagozdzon, A.; Sicinska, E.; Geng, Y.; Yu, Q.; Bhattacharya, S.; Bronson, R. T.; Akashi, K.; Sicinski, P. : Mouse development and cell proliferation in the absence of D-cyclins. Cell 118: 477-491, 2004. 2. Inaba, T.; Matsushime, H.; Valentine, M.; Roussel, M. F.; Sherr, C. J.; Look, A. T. : Genomic organization, chromosomal localization, and independent expression of human cyclin D genes. Genomics 13: 565-574, 1992. 3. Xiong, Y.; Menninger, J.; Beach, D.; Ward, D. C. : Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics 13: 575-584, 1992.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 32826 MW
- Observed Molecular Weight
- 55 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- G1/S-specific cyclin-D2
- Subcellular Location
- Nucleus . Cytoplasm . Membrane . Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. .
- Sequence Similarities
- Belongs to the cyclin family. Cyclin D subfamily.
- Protein Name
- G1/S-specific cyclin-D2
- Isotype
- Mouse IgG2a
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
