Anti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4)

About this product

Boster Bio Anti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4) catalog # MA1044. Tested in IHC, WB applications. This antibody reacts with Human, Rabbit.

Specifications

Catalog number

MA1044

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.

Synonyms

Gelsolin; Actin-depolymerizing factor; ADF; Brevin; Gsn

Gene Name

GSN

Gene ID

Human (2934), Mouse (227753), Rat (296654)

UniProt

Q68FP1

Host

Mouse

Reactivity

Human, Rabbit

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human plasma gelsolin.

Clonality

Monoclonal

Clone

Clone: GS-2C4

Tissue Specificity

GSN: Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.

Applications

IHC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping) . It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis (By similarity) . .

References & Citations

1. Kwiatkowski, D. J.; Ozelius, L.; Schuback, D.; Gusella, J.; Breakefield, X. O. : The gelsolin (GSN) cDNA clone, from 9q32-34, identifies BclI and StuI RFLPs. Nucleic Acids Res. 17: 4425 only, 1989. 2. Kwiatkowski, D. J.; Westbrook, C. A.; Bruns, G. A. P.; Morton, C. C. : Localization of gelsolin proximal to ABL on chromosome 9. Am. J. Hum. Genet. 42: 565-572, 1988. 3. Kwiatkowski, D. J.; Stossel, T. P.; Orkin, S. H.; Mole, J. E.; Colten, H. R.; Yin, H. L. : Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. Nature 323: 455-458, 1986.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

86068 MW

Observed Molecular Weight

90 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Gelsolin

Subcellular Location

Isoform 1: Secreted .

Sequence Similarities

Belongs to the villin/gelsolin family.

Protein Name

Gelsolin

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.