Anti-GFAP Antibody (Monoclonal, G-A-5)

About this product

Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.

Specifications

Catalog number

MA1045

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

Synonyms

Glial fibrillary acidic protein; GFAP; Gfap

Gene Name

GFAP

Gene ID

Human (2670), Mouse (14580), Rat (24387)

UniProt

P47819

Host

Mouse

Reactivity

Human, Mouse, Pig, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

GFAP from pig spinal cord.

Clonality

Monoclonal

Clone

Clone: G-A-5

Tissue Specificity

Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment. .

Applications

IF, IHC, IHC-F, WB

Field of Research

Cell Adhesion Proteins, Cell Type Marker, Cell Type Markers, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Neural Stem Cells, Neuroscience, Signal Transduction, Stem Cells, Tags & Cell Markers

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

References & Citations

1. Brenner, M.; Johnson, A. B.; Boespflug-Tanguy, O.; Rodriguez, D.; Goldman, J. E.; Messing, A. : Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. Nature Genet. 27: 117-120, 2001. 2. Rodriguez, D.; Gauthier, F.; Bertini, E.; Bugiani, M.; Brenner, M.; N'guyen, S.; Goizet, C.; Gelot, A.; Surtees, R.; Pedespan, J.-M.; Hernandorena, X.; Troncoso, M.; Uziel, G.; Messing, A.; Ponsot, G.; Pham-Dinh, D.; Dautigny, A.; Boespflug-Tanguy, O. : Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation. Am. J. Hum. Genet. 69: 1134-1140, 2001. Note: Erratum: Am. J. Hum. Genet. 69: 1413 only, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

49957 MW

Observed Molecular Weight

50 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Glial fibrillary acidic protein

Subcellular Location

Cytoplasm . Associated with intermediate filaments. .

Sequence Similarities

Belongs to the intermediate filament family.

Protein Name

Glial fibrillary acidic protein

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.