- Catalog number
- MA1049
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.
- Synonyms
- 60 kDa heat shock protein, mitochondrial;60 kDa chaperonin; Chaperonin 60; CPN60; HSP-65; Heat shock protein 60; HSP-60; Hsp60; Mitochondrial matrix protein P1; Hspd1; Hsp60
- Gene Name
- HSPD1
- Gene ID
- Human (3329), Mouse (15510), Rat (63868)
- UniProt
- P63039
- Host
- Mouse
- Reactivity
- Chicken, Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Recombinant human heat shock protein 60 (HSP60)
- Clonality
- Monoclonal
- Clone
- Clone: LK1
- Tissue Specificity
- Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles. .
- Applications
- IHC, WB
- Field of Research
- Chaperones, Heat Shock Proteins, Mitochondria, Organelles, Protein Trafficking, Signal Transduction, Subcellular Markers, Tags & Cell Markers
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
- References & Citations
- 1. Cheng, M. Y.; Hartl, F.-U.; Martin, J.; Pollock, R. A.; Kalousek, F.; Neupert, W.; Hallberg, E. M.; Hallberg, R. L.; Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989. 2. Hansen, J. J.; Bross, P.; Westergaard, M.; Nielsen, M. N.; Eiberg, H.; Borglum, A. D.; Mogensen, J.; Kristiansen, K.; Bolund, L.; Gregersen, N. : Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a biional promoter. Hum. Genet. 112: 71-77, 2003. Note: Erratum: Hum. Genet. 112: 436 only, 2003. 3. Zanin-Zhorov, A.; Cahalon, L.; Tal, G.; Margalit, R.; Lider, O.; Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 60955 MW
- Observed Molecular Weight
- 65 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- 60 kDa heat shock protein, mitochondrial
- Subcellular Location
- Mitochondrion matrix .
- Sequence Similarities
- Belongs to the chaperonin (HSP60) family.
- Protein Name
- 60 kDa heat shock protein, mitochondrial
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
