Anti-Involucrin Ivl Antibody (Monoclonal, SY5)

About this product

Boster Bio Anti-Involucrin Ivl Antibody (Monoclonal, SY5) catalog # MA1053. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Pig.

Specifications

Catalog number

MA1053

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated.

Synonyms

Involucrin; Ivl

Gene Name

IVL

Gene ID

Human (3713), Mouse (16447), Rat (60583)

UniProt

P48998

Host

Mouse

Reactivity

Human, Pig

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human involucrin.

Clonality

Monoclonal

Clone

Clone: SY5

Tissue Specificity

Keratinocytes of epidermis and other stratified squamous epithelia.

Applications

IHC, ICC, WB

Field of Research

Cancer, Cytoskeleton/ECM, Extracellular Matrix, Keratin, Signal Transduction, Structures, Tumor Biomarkers

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

References & Citations

1. Eckert, R. L.; Green, H. : Structure and evolution of the human involucrin gene. Cell 46: 583-589, 1986. 2. Djian, P.; Delhomme, B.; Green, H. : Origin of the polymorphism of the involucrin gene in Asians. Am. J. Hum. Genet. 56: 1367-1372, 1995.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

67021 MW

Observed Molecular Weight

68 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Involucrin

Subcellular Location

Cytoplasm. Constituent of the scaffolding of the cornified envelope.

Sequence Similarities

Belongs to the insulin family.

Protein Name

Involucrin

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.