- Catalog number
- MA1054
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1, beta-1 and gamma-1, formerly called beta-2 (LAMA2) . This gene is over 260,000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.
- Synonyms
- Laminin subunit beta-2; Laminin chain B3; Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; Laminin-9 subunit beta; S-laminin subunit beta; S-LAM beta; Lamb2
- Gene Name
- LAMA1; LAMA2; LAMB1; LAMB2
- Gene ID
- Human (284217), Mouse (16772), Rat (316758), Human (3908), Mouse (16773), Rat (309368), Human (3912), Mouse (16777), Rat (298941), Human (3913), Mouse (16779), Rat (25473)
- UniProt
- P15800
- Host
- Mouse
- Reactivity
- Human, Pig
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Human laminin.
- Clonality
- Monoclonal
- Clone
- Clone: LAM-89
- Tissue Specificity
- Found in the basement membranes (major component) . S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.
- Applications
- IHC
- Field of Research
- Angiogenesis, Cardiovascular, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Intermediate Filaments, Neuroscience, Signal Transduction
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
- References & Citations
- 1. Zhang, X.; Vuolteenaho, R.; Tryggvason, K. : Structure of the human laminin alpha-2-chain gene (LAMA2), which is affected in congenital muscular dystrophy. J. Biol. Chem. 271: 27664-27669, 1996. 2. Ehrig, K.; Leivo, I.; Argraves, W. S.; Ruoslahti, E.; Engvall, E. : Merosin, a tissue-specific basement membrane protein, is a laminin-like protein. Proc. Nat. Acad. Sci. 87: 3264-3268, 1990. 3. Arahata, K.; Hayashi, Y. K.; Mizuno, Y.; Yoshida, M.; Ozawa, E. : Dystrophin-associated glycoprotein and dystrophin co-localisation at sarcolemma in Fukuyama congenital muscular dystrophy. (Letter) Lancet 342: 623-624, 1993.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 196474 MW
- Observed Molecular Weight
- 68 kDa
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, feline, pig
- Gene Name Synonym
- Laminin subunit beta-2
- Subcellular Location
- Secreted, extracellular space, extracellular matrix, basement membrane.
- Sequence Similarities
- Contains 17 laminin EGF-like domains.
- Protein Name
- Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
