Anti-Mcm7 Antibody (Monoclonal, DCS-141)

About this product

Boster Bio Anti-Mcm7 Antibody (Monoclonal, DCS-141) catalog # MA1058. Tested in ICC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

MA1058

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Initiation of DNA replication is a complex process involving the concerted action of many proteins. The chromosome 7-linked gene referred to by Nakatsuru et al. as MCM2 has been designated MCM7. The MCM2-7 complex is comprised of 6 subunits, MCM2 through MCM7, and is a ring-shaped heterohexameric ATPase involved in DNA replication. MCM7 is located in 7q21.3-q22.1.

Synonyms

DNA helicase ;3.6.4.12 ; Mcm7 ; rCG_55961

Gene Name

MCM7

Gene ID

Human (4176), Mouse (17220), Rat (288532)

UniProt

Q6AYN8

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Recombinant human Mcm7.

Clonality

Monoclonal

Clone

Clone: DCS-141

Applications

ICC, WB

Field of Research

DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

MCM7: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once percell cycle' DNA replication initiation and elongation ineukaryotic cells. The active ATPase sites in the MCM2-7 ring areformed through the interaction surfaces of two neighboringsubunits such that a critical structure of a conserved argininefinger motif is provided in trans relative to the ATP-binding siteof the Walker A box of the adjacent subunit. The six ATPase activesites, however, are likely to contribute differentially to thecomplex helicase activity. Required for S-phase checkpointactivation upon UV-induced damage.

References & Citations

1. Nakatsuru, S.; Sudo, K.; Nakamura, Y. : Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication. Cytogenet. Cell Genet. 68: 226-230, 1995. 2. Pacek, M.; Tutter, A. V.; Kubota, Y.; Takisawa, H.; Walter, J. C. : Localization of MCM2-7, Cdc45, and GINS to the site of DNA unwinding during eukaryotic DNA replication. Molec. Cell 21: 581-587, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

81063 MW

Observed Molecular Weight

42 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

DNA helicase

Subcellular Location

Nucleus .

Sequence Similarities

Belongs to the MCM family.

Protein Name

DNA replication licensing factor MCM7

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.