Anti-P-GlycoProtein (MDR) ABCB1 Antibody (Monoclonal, F4)

About this product

Boster Bio Anti-P-GlycoProtein (MDR) ABCB1 Antibody (Monoclonal, F4) catalog # MA1060. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

MA1060

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

P-Glycoprotein, , also known as Multidrug Resistance 1 (MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.

Synonyms

Multidrug resistance protein 1A;3.6.3.44; ATP-binding cassette sub-family B member 1A; MDR1A; Multidrug resistance protein 3; P-glycoprotein 3; Abcb1a; Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3

Gene Name

Abcb1a

Gene ID

Human (5243), Mouse (18671), Rat (170913)

UniProt

P08183

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Clonality

Monoclonal

Clone

Clone: F4

Tissue Specificity

ABCB1: Expressed in liver, kidney, small intestineand brain.

Applications

IHC, ICC, WB

Field of Research

ATPases, Cancer, Lipid Metabolism, Metabolism, Nuclear Import/Export, Plasma Membrane, Protein Trafficking, Signal Transduction

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. .

References & Citations

1. Callen, D. F.; Baker, E.; Simmers, R. N.; Seshadri, R.; Roninson, I. B. : Localization of the human multiple drug resistance gene, MDR1, to 7q21.1. Hum. Genet. 77: 142-144, 1987. 2. de Lannoy, I. A. M.; Silverman, M. : The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin. Biochem. Biophys. Res. Commun. 189: 551-557, 1992.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

140647 MW

Observed Molecular Weight

200 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

DNA helicase

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Sequence Similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Protein Name

DNA replication licensing factor MCM7

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.