Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1)

About this product

Boster Bio Anti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) catalog # MA1061. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rabbit, Rat.

Specifications

Catalog number

MA1061

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mucin 5AC  (Muc5AC)   is  a  protein  that  in  humans  is  encoded  by  the  MUC5AC  gene.  Using  real-time  RT-PCR,   Moehle  et  al.  (2006)   found  that  MUC5AC  was  highly  expressed  in  adult  trachea  and  stomach.  Expression  was  lower  in  adult  lung  and  weak  in  fetal  lung,   and  no  expression  was  detected  in  other  tissues.  This  gene  has  been  linked  to  mucus  hypersecretion  in  the  pulmonary  tracts  and  associated  to  chronic  obstructive  pulmonary  disease.  Moreover,   Muc5ac  is  identified  as  a    and  critical  mediator  of  resistance  during  intestinal  nematode  infection.

Synonyms

Mucin-5AC ; MUC-5AC ; Gastric mucin ; Lewis B blood group antigen ; LeB ; Major airway glycoprotein ; Mucin-5 subtype AC, tracheobronchial; Tracheobronchial mucin ; TBM ; MUC5AC ; MUC5

Gene Name

MUC5B

Gene ID

Human (4586), Mouse (17833), Rat (682837)

UniProt

P98088

Host

Mouse

Reactivity

Human, Rabbit, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Mucin from human ovarian cyst fluid.

Clonality

Monoclonal

Clone

Clone: 45M1

Tissue Specificity

Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .

Applications

IHC, ICC, WB

Field of Research

Cancer, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Tumor Antigens, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.

References & Citations

1. Hasnain, S. Z., Evans, C. M., Roy, M., Gallagher, A. L., Kindrachuk, K. N., Barron, L., Dickey, B. F., Wilson, M. S., Wynn, T. A., Grencis, R. K., Thornton, D. J. Muc5ac: a critical component mediating the rejection of enteric nematodes. J. Exp. Med. 208: 893-900, 2011. 2. Moehle, C., Ackermann, N., Langmann, T., Aslanidis, C., Kel, A., Kel-Margoulis, O., Schmitz-Madry, A., Zahn, A., Stremmel, W., Schmitz, G. Aberrant intestinal expression and allelic variants of mucin genes associated with inflammatory bowel disease. J. Molec. Med. 84: 1055-1066, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

585570 MW

Observed Molecular Weight

200 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Mucin-5AC

Subcellular Location

Secreted .

Sequence Similarities

Contains 1 CTCK (C-terminal cystine knot-like) domain.

Protein Name

Mucin-5AC

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.