- Catalog number
- MA1070
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL) . It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.
- Synonyms
- Neurofilament light polypeptide; NF-L;68 kDa neurofilament protein; Neurofilament triplet L protein; Nefl; Nf68, Nfl
- Gene Name
- NEFL
- Gene ID
- Human (4747), Mouse (18039), Rat (83613)
- UniProt
- P19527
- Host
- Mouse
- Reactivity
- Human, Mouse, Pig, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Pig spinal cord.
- Clonality
- Monoclonal
- Clone
- Clone: NR4
- Tissue Specificity
- Muscle specific. Located in the thin filament of striated muscle.
- Applications
- IHC, IHC-F, WB
- Field of Research
- Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Developmental Biology, Intermediate Filaments, Lineage Markers, Lineage Specification, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction, Stem Cells
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
- References & Citations
- 1. Zhu, Q.; Couillard-Despres, S.; Julien, J.-P. : Delayed maturation of regenerating myelinated axons in mice lacking neurofilaments. Exp. Neurol. 148: 299-316, 1997. 2. Jordanova, A.; De Jonghe, P.; Boerkoel, C. F.; Takashima, H.; De Vriendt, E.; Ceuterick, C.; Martin, J.-J.; Butler, I. J.; Mancias, P.; Papasozomenos, S. C.; Terespolsky, D.; Potocki, L.; Brown, C. W.; Shy, M.; Rita, D. A.; Tournev, I.; Kremensky, I.; Lupski, J. R.; Timmerman, V. : Mutations in the neurofilament light chain gene (NEFL) cause early onset severe Charcot-Marie-Tooth disease. Brain 126: 590-597, 2003. 3. Previtali, S. C.; Zerega, B.; Sherman, D. L.; Brophy, P. J.; Dina, G.; King, R. H. M.; Salih, M. M.; Feltri, L.; Quattrini, A.; Ravazzolo, R.; Wrabetz, L.; Monaco, A. P.; Bolino, A. : Myotubularin-related 2 protein phosphatase and neurofilament light chain protein, both mutated in CMT neuropathies, interact in peripheral nerve. Hum. Molec. Genet. 12: 1713-1723, 2003.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 61335 MW
- Observed Molecular Weight
- 72 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Neurofilament light polypeptide
- Subcellular Location
- Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.
- Sequence Similarities
- Belongs to the intermediate filament family.
- Protein Name
- Neurofilament light polypeptide
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
