- Catalog number
- MA1072
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Nitric Oxide Synthase 1 (NOS1, neuronal NOS, nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.
- Synonyms
- Nitric oxide synthase, brain;1.14.13.39; BNOS; Constitutive NOS; NC-NOS; N
- Gene Name
- NOS1
- Gene ID
- Human (4842), Mouse (18125), Rat (24598)
- UniProt
- P29476
- Host
- Mouse
- Reactivity
- Goat, Human, Pig, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Recombinant neuronal NOS fragment (amino acids 1-181) from rat brain.
- Clonality
- Monoclonal
- Clone
- Clone: NOS-B1
- Tissue Specificity
- Isoform N-NOS-1 is expressed in brain and colorectum. Found in the Auerbach's plexus of the enteric nervous system. Isoform PNNOS is expressed in the penis, urethra, prostate, and skeletal muscle, and coexists with the cerebellar nnos in the pelvic plexus, bladder and liver, and is detectable in the cerebellum. .
- Applications
- WB
- Field of Research
- Alzheimer's Disease, Cell Adhesion Proteins, Cell Type Marker, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Inhibitory transmitter for non-adrenergic and non-cholinergic nerves in the colorectum. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Inhibitory transmitter for non- adrenergic and non-cholinergic nerves in the colorectum.
- References & Citations
- 1. Brenman, J. E.; Chao, D. S.; Xia, H.; Aldape, K.; Bredt, D. S. : Nitric oxide synthase complexed with dystrophin and absent from skeletal muscle sarcolemma in Duchenne muscular dystrophy. Cell 82: 743-752, 1995. 2. Wang, Y.; Goligorsky, M. S.; Lin, M.; Wilcox, J. N.; Marsden, P. A. : A novel, testis-specific mRNA transcript encoding an NH (2) -terminal truncated nitric-oxide synthase. J. Biol. Chem. 272: 11392-11401, 1997. 3. Xie, J.; Roddy, P.; Rife, T. K.; Murad, F.; Young, A. P. : Two closely linked but separable promoters for human neuronal nitric oxide synthase gene transcription. Proc. Nat. Acad. Sci. 92: 1242-1246, 1995. 4. Kharazia, V. N.; Schmidt, H. H. H. W.; Weinberg, R. J. : Type I nitric oxide synthase fully accounts for NADPH-diaphorase in rat striatum, but not cortex. Neuroscience 62: 983-987, 1994.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 160559 MW
- Observed Molecular Weight
- 200 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Nitric oxide synthase, brain
- Subcellular Location
- Cell membrane, sarcolemma ; Peripheral membrane protein . Cell projection, dendritic spine . In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex (By similarity) . In neurons, enriched in dendritic spines. .
- Sequence Similarities
- Belongs to the NOS family.
- Protein Name
- Nitric oxide synthase, brain
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
