Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)

About this product

Boster Bio Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) catalog # MA1074. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

MA1074

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

P16 (INK4A), also known as cyclin-dependent kinase inhibitor 2A (CDKN2A), or multiple tumor suppressor 1 (MTS1) . The p16 gene (CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma.

Synonyms

Cyclin-dependent kinase inhibitor 2A ; Cyclin-dependent kinase 4 inhibitor A; CDK4I; p16-INK4a; p16; p16-INK4; Cdkn2a ; P16ink4a

Gene Name

CDKN2A

Gene ID

Human (1029), Mouse (12578), Rat (25163)

UniProt

Q9R0Z3

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Recombinant human p16 protein.

Clonality

Monoclonal

Clone

Clone: DCS-50

Tissue Specificity

Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. .

Applications

IHC, ICC, WB

Field of Research

Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling, Oncoproteins/Suppressors, p53 Pathway, Transcription, Tumor Suppressors

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity) . .

References & Citations

1. Stone, S.; Jiang, P.; Dayananth, P.; Tavtigian, S. W.; Katcher, H.; Parry, D.; Peters, G.; Kamb, A. : Complex structure and regulation of the p16 (MTS1) locus. Cancer Res. 55: 2988-2994, 1995. 2. Monzon, J.; Liu, L.; Brill, H.; Goldstein, A. M.; Tucker, M. A.; From, L.; McLaughlin, J.; Hogg, D.; Lassam, N. J. : CDKN2A mutations in multiple primary melanomas. New Eng. J. Med. 338: 879-887, 1998. 3. Bartsch, D.; Shevlin, D. W.; Tung, W. S.; Kisker, O.; Wells, S. A., Jr.; Goodfellow, P. J. : Frequent mutations of CDKN2 in primary pancreatic adenocarcinomas. Genes Chromosomes Cancer 14: 189-195, 1995

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

17366 MW

Observed Molecular Weight

200 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Cyclin-dependent kinase inhibitor 2A

Subcellular Location

Cytoplasm . Nucleus .

Sequence Similarities

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Protein Name

Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.