Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)

About this product

Boster Bio Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) catalog # MA1081. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Specifications

Catalog number

MA1081

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Synonyms

Keratin, type II cytoskeletal 1;67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1; KRT1; KRTA

Gene Name

KRT1

Gene ID

3848

UniProt

P04264

Host

Mouse

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Mixture of several monoclonal cytokeratin clones.

Clonality

Monoclonal

Clone

Clone: C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2

Tissue Specificity

The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.

Applications

IHC, WB

Field of Research

Cytoskeleton/ECM, Intermediate Filaments, Microtubules, Signal Transduction

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1) . In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. .

References & Citations

1. Rastaldi MP et al. Glomerular podocytes contain neuron-like functional synaptic vesicles. FASEB J 20:976-8 (2006) . 2. Wen J et al. Use of superparamagnetic microbeads in tracking subretinal injections. Mol Vis 11:256-62 (2005) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

66039 MW

Observed Molecular Weight

45-60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Keratin, type II cytoskeletal 1

Subcellular Location

Cell membrane . Located on plasma membrane of neuroblastoma NMB7 cells.

Sequence Similarities

Belongs to the intermediate filament family.

Protein Name

Keratin, type II cytoskeletal 1

Isotype

Mouse IgG2a/IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.