Anti-Spectrin (Alpha And Beta) SPTB Antibody (Monoclonal, SB-SP1)

About this product

Boster Bio Anti-Spectrin (Alpha And Beta) SPTB Antibody (Monoclonal, SB-SP1) catalog # MA1090. Tested in WB applications. This antibody reacts with Human.

Specifications

Catalog number

MA1090

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5.

Synonyms

Erythroid spectrin beta ; Sptb ; LOC314251

Gene Name

SPTA1; SPTB

Gene ID

Human (6708), Mouse (20739), Rat (289257), Human (6710), Mouse (20741), Rat (314251)

UniProt

Q6XDA0

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human erythrocyte spectrin.

Clonality

Monoclonal

Clone

Clone: SB-SP1

Applications

WB

Field of Research

Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Plasma Membrane, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

SPTA1: Spectrin is the major constituent of the cytoskeletalnetwork underlying the erythrocyte plasma membrane. It associateswith band 4.1 and actin to form the cytoskeletal superstructure ofthe erythrocyte plasma membrane.|SPTB: Spectrin is the major constituent of the cytoskeletalnetwork underlying the erythrocyte plasma membrane. It associateswith band 4.1 and actin to form the cytoskeletal superstructure ofthe erythrocyte plasma membrane.

References & Citations

1. Watkins, P. C.; Eddy, R.; Forget, B. G.; Chang, J. G.; Rochelle, R.; Shows, T. B. : Assignment of a non-erythroid spectrin gene to human chromosome 2. (Abstract) Am. J. Hum. Genet. 43: A161, 1988. 2. Ikeda, Y.; Dick, K. A.; Weatherspoon, M. R.; Gincel, D.; Armbrust, K. R.; Dalton, J. C.; Stevanin, G.; Durr, A.; Zuhlke, C.; Burk, K.; Clark, H. B.; Brice, A.; Rothstein, J. D.; Schut, L. J.; Day, J. W.; Ranum, L. P. W. : Spectrin mutations cause spinocerebellar ataxia type 5. Nature Genet. 38: 184-190, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

246329 MW

Observed Molecular Weight

60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Spectrin, alpha, erythrocytic 1 (elliptocytosis 2) ; spectrin, beta, erythrocytic

Subcellular Location

SPTA1: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.|Spta1: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.|SPTB: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.|Sptb: Cytoplasm, cytoskeleton. Cytoplasm, cellcortex.

Sequence Similarities

Belongs to the spectrin family.

Protein Name

Spectrin alpha chain, erythrocytic 1; Spectrin beta chain, erythrocytic

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.