Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38)

About this product

Boster Bio Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38) catalog # MA1091. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Specifications

Catalog number

MA1091

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Synaptophysin (SYP) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Ozcelik et al. (1990) concluded that the gene has 7 exons distributed over about 20 kb. SYP mapped the SYP locus to Xp11.23-p11.22. Regionalization of the gene on the X chromosome was also done with hamster/human hybrid cells in which various portions of the human X chromosome were present.

Synonyms

Synaptophysin; Major synaptic vesicle protein p38; Syp

Gene Name

SYP

Gene ID

Human (6855), Mouse (20977), Rat (24804)

UniProt

P07825

Host

Mouse

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Rat retina synaptosome.

Clonality

Monoclonal

Clone

Clone: SVP-38

Tissue Specificity

Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

Applications

IHC, WB

Field of Research

Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Plasma Membrane, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity) . .

References & Citations

1. McMahon, H. T.; Bolshakov, V. Y.; Janz, R.; Hammer, R. E.; Siegelbaum, S. A.; Sudhof, T. C. : Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release. Proc. Nat. Acad. Sci. 93: 4760-4764, 1996. 2. Cremin, S. M.; Greer, W. L.; Bodok-Nutzati, R.; Schwartz, M.; Peacocke, M.; Siminovitch, K. A. : Linkage of Wiskott-Aldrich syndrome with three marker loci, DXS426, SYP and TFE3, which map to the Xp11.3-p11.22 region. Hum. Genet. 92: 250-253, 1993. 3. Ozcelik, T.; Lafreniere, R. G.; Archer, B. T., III; Johnston, P. A.; Willard, H. F.; Francke, U.; Sudhof, T. C. : Synaptophysin: structure of the human gene and assignment to the X chromosome in man and mouse. Am. J. Hum. Genet. 47: 551-561, 1990.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33311 MW

Observed Molecular Weight

60 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Synaptophysin

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.

Sequence Similarities

Belongs to the synaptophysin/synaptobrevin family.

Protein Name

Synaptophysin

Isotype

Mouse IgG1

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.