- Catalog number
- MA1092
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Talin is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Talin serves a function of linking vinculin to the integrins, and, thus, the cytoskeleton to extracellular matrix (ECM) receptors. It has a mass of 270 kD and shares 23% N-terminal identity with ezrin, which has similar functions. TLN gene is mapped to 9p.
- Synonyms
- Protein Tln2 ; Tln2
- Gene Name
- TLN1; TLN2
- Gene ID
- Human (7094), Mouse (21894), Rat (313494), Human (83660), Mouse (70549), Rat (315776)
- UniProt
- D3ZA84
- Host
- Mouse
- Reactivity
- Chicken, Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Purified chicken gizzard talin.
- Clonality
- Monoclonal
- Clone
- Clone: 8d4
- Tissue Specificity
- Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
- Applications
- WB
- Field of Research
- Actin Assembly, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- TLN1: Probably involved in connections of major cytoskeletalstructures to the plasma membrane. High molecular weightcytoskeletal protein concentrated at regions of cell-substratumcontact and, in lymphocytes, at cell-cell contacts (Bysimilarity) . |TLN2: As a major component of focal adhesion plaques thatlinks integrin to the actin cytoskeleton, may play an importantrole in cell adhesion. Recruits PIP5K1C to focal adhesion plaquesand strongly activates its kinase activity (By similarity) .
- References & Citations
- 1. Rees, D. J. G.; Ades, S. E.; Singer, S. J.; Hynes, R. O. : Sequence and domain structure of talin. Nature 347: 685-689, 1990. 2. Gilmore, A. P.; Ohanian, V.; Spurr, N. K.; Critchley, D. R. : Localisation of the human gene encoding the cytoskeletal protein talin to chromosome 9p. Hum. Genet. 96: 221-224, 1995.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 271684 MW
- Observed Molecular Weight
- 272 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Talin 1; talin 2
- Subcellular Location
- TLN1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface Cell junction, focaladhesion Note: Colocalizes with LAYN at the membraneruffles. Localized preferentially in focal adhesions thanfibrillar adhesions (By similarity) . |Tln1: Cell projection, ruffle membrane Cytoplasm, cytoskeleton Cell surface |TLN2: Cell junction, focal adhesion. Celljunction, synapse. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Note: Focal adhesionplaques and synapses.|Tln2: Cell junction, focal adhesion Cell junction, synapse Cell membrane Cytoplasm, cytoskeleton Note: Focaladhesion plaques and synapses.
- Sequence Similarities
- Contains 1 FERM domain.
- Protein Name
- Talin-1; Talin-2
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
