- Catalog number
- MA1114
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.
- Synonyms
- Golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Predicted) ; Protein Gga3 ; Gga3 ; Gga3_predicted ; rCG_32821
- Gene Name
- GGA1; GGA2; GGA3
- Gene ID
- 360658
- UniProt
- D3ZHG8
- Host
- Mouse
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Synthetic gamma-tubulin peptide (amino acids 38-53), conjugated to KLH.
- Clonality
- Monoclonal
- Clone
- Clone: GTU-88
- Tissue Specificity
- GGA1: Ubiquitously expressed.
- Applications
- ICC, WB
- Field of Research
- Golgi Proteins, Protein Trafficking, Signal Transduction
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- GGA1: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.|GGA2: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.|GGA3: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.
- References & Citations
- 1. Rommens, J. M.; Durocher, F.; McArthur, J.; Tonin, P.; LeBlanc, J.-F.; Allen, T.; Samson, C.; Ferri, L.; Narod, S.; Morgan, K.; Simard, J. : Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21. Genomics 28: 530-542, 1995. 2. Zheng, Y.; Jung, M. K.; Oakley, B. R. : Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65: 817-823, 1991. 3. Stearns, T.; Evans, L.; Kirschner, M. : Gamma-tubulin is a highly conserved component of the centrosome. Cell 65: 825-836, 1991.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 71715 MW
- Observed Molecular Weight
- 55 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3
- Subcellular Location
- GGA1: Golgi apparatus, trans-Golgi networkmembrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.|Gga1: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA2: Golgi apparatus, trans-Golgi networkmembrane |Gga2: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane |GGA3: Golgi apparatus, trans-Golgi networkmembrane |Gga3: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane
- Sequence Similarities
- Belongs to the GGA protein family.
- Protein Name
- ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
