- Catalog number
- MA1116
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.
- Synonyms
- Fibronectin; FN; Anastellin; Fn1
- Gene Name
- FN1
- Gene ID
- Human (2335), Mouse (14268), Rat (25661)
- UniProt
- P04937
- Host
- Mouse
- Reactivity
- Chicken, Human, Mouse
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- Fibronectin from human plasma.
- Clonality
- Monoclonal
- Clone
- Clone: FN-15
- Tissue Specificity
- Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
- Applications
- IHC, ICC, WB
- Field of Research
- Actin, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction
- Purification
- Ascites
- Concentration
- Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
- Function
- Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity) . .
- References & Citations
- 1. Saad S, Gottieb DJ, Bradstock KF, Overall CM, Bendall Lj. Cancer cell-associated fibronectin induces release of matrix metalloproteinase-2 from normal fibroblasts. Cancer Res. 2002 Jan 1;62 (1) :283-9. 2. Saito N, Mitsuhashi M, Hayashi T, Narumo C, Nagata H, Soyama K, Kameoka S, Harumiya S, Fujimoto D. Inhibition of hepatic metastasis in mice treated with cell-binding domain of human fibronectin and angiogenesis inhibitor TNP-470. Int J Clin Oncol.2001 Oct;6 (5) :215-20. 3. Chassagne C, Adamy C, Ratajczak P, Gingras B, Teiger E, Planus E, Oliviero P, Pappaport L, Samuel JL, Meloche S. Angiotensin II AT (2) receptor inhibits smooth muscle cell migration via fibronectin cell production and binding. Am J Physiol Cell Physiol. 2002 Apr;282 (4) :C654-64.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 272511 MW
- Observed Molecular Weight
- 42 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Fibronectin
- Subcellular Location
- Secreted, extracellular space, extracellular matrix.
- Sequence Similarities
- Contains 12 fibronectin type-I domains.
- Protein Name
- Fibronectin
- Isotype
- Mouse IgG1
- Contents
- Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
