Anti-Alkaline Phosphatase/ALPL Antibody Picoband®

About this product

Boster Bio Anti-Alkaline Phosphatase/ALPL Antibody catalog # PA1004-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1004-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Alkaline phosphatase, tissue-nonspecific isozyme is an enzyme that in humans is encoded by the ALPL gene. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific) . The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Liver/bone/kidney alkaline phosphatase, also known as tissue-nonspecific alkaline phosphatase, acts physiologically as a lipid-anchored phosphoethanolamine (PEA) and pyridoxal-5-prime-phosphate (PLP) ectophosphatase Mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked ly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects.

Synonyms

Alkaline phosphatase, tissue-nonspecific isozyme; AP-TNAP; TNSALP;3.1.3.1; Alkaline phosphatase liver/bone/kidney isozyme; Alpl

Gene Name

ALPL

Gene ID

P08289

UniProt

P08289

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of rat Alkaline Phosphatase, different from the related mouse sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with highest expression in brain. .

Applications

WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Kinases/Phosphatases, Tags & Cell Markers, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This isozyme may play a role in skeletal mineralization.

References & Citations

1. Weiss MJ, Henthorn PS, Lafferty MA, Slaughter C, Raducha M, Harris H (Oct 1986) . Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase. Proc Natl Acad Sci U S A 83 (19) : 7182–6. 2. Swallow DM, Povey S, Parkar M, Andrews PW, Harris H, Pym B, Goodfellow P (May 1988) . Mapping of the gene coding for the human liver/bone/kidney isozyme of alkaline phosphatase to chromosome 1. Ann Hum Genet 50 (Pt 3) : 229–35. 3. Entrez Gene: ALPL alkaline phosphatase, liver/bone/kidney 4. Fedde, K. N., Whyte, M. P. Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5-prime-phosphate ectophosphatase: normal and hypophosphatasia fibroblast study. Am. J. Hum. Genet. 47: 767-775, 1990.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

57659 MW

Observed Molecular Weight

57 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Alkaline phosphatase, tissue-nonspecific isozyme

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Sequence Similarities

Contains 12 fibronectin type-I domains.

Protein Name

Alkaline phosphatase, tissue-nonspecific isozyme

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.