Anti-CD40L/CD40LG Antibody Picoband®

About this product

Boster Bio Anti-CD40L/CD40LG Antibody catalog # PA1020. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1020

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells. CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Synonyms

CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40LG; CD40L, TNFSF5, TRAP

Gene Name

CD40LG

Gene ID

959

UniProt

P29965

Host

Rabbit

Reactivity

Human, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CD40L, different from the related mouse sequence by three amino acids, rat sequence by four amino acids.

Clonality

Polyclonal

Tissue Specificity

Specifically expressed on activated CD4+ T- lymphocytes.

Applications

IHC, WB

Field of Research

Adaptive Immunity, Atherosclerosis, Cardiovascular, Cytokines, Immunoglobulins, Immunology, Innate Immunity, Platelets, T Cells, TNF Superfamily, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. .

References & Citations

1. Allen, R.C.; Armitage, R.J.; Conley, M.E.; Rosenblatt, H.; Jenkins, N.A.; Copeland, N.G.; Bedell, M.A.; Edelhoff, S.; Disteche, C.M.; Simoneaux, D.K.; Fanslow, W.C.; Belmont, J.; Spriggs, M.K.:CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome. Science 259: 990-993, 1993. 2. Cipollone, F.; Ferri, C.; Desideri, G.; Paloscia, L.; Materazzo, G.; Mascellanti, M.; Fazia, M.; Iezzi, A.; Cuccurullo, C.; Pini, B.; Bucci, M.; Santucci, A.; Cuccurullo, F.; Mezzetti, A.:Preprocedural level of soluble CD40L is predictive of enhanced inflammatory response and restenosis after coronary angioplasty. Circulation 108: 2776-2782, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29274 MW

Observed Molecular Weight

29 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

CD40 ligand

Subcellular Location

Cell membrane; Single-pass type II membrane protein.

Sequence Similarities

Belongs to the tumor necrosis factor family.

Protein Name

CD40 ligand (CD40-L)

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.