Anti-DUT Antibody Picoband®

About this product

Boster Bio Anti-DUT Antibody catalog # PA1030-1. Tested in ICC/IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1030-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35% identity with the E.coli dUTPase and 53% identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92% identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.

Synonyms

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTPase;3.6.1.23; dUTP pyrophosphatase; DUT

Gene Name

DUT

Gene ID

1854

UniProt

P33316

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DUT, different from the related rat and mouse sequences by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level) . Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level) . .

Applications

ICC/IF, IHC, WB

Field of Research

DNA/Nucleotides, DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. .

References & Citations

1. Studebaker AW, Lafuse WP, Kloesel R, Williams MV.Modulation of human dUTPase using small interfering RNA. Biochem Biophys Res Commun. 2005 Feb 4; 327 (1) :306-10. 2. McIntosh EM, Ager DD, Gadsden MH, Haynes RH.Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc Natl Acad Sci U S A. 1992 Sep 1; 89 (17) :8020-4. 3. Chu R, Lin Y, Rao MS, Reddy JK.Cloning and identification of rat deoxyuridine triphosphatase as an inhibitor of peroxisome proliferator-activated receptor alpha. J Biol Chem. 1996 Nov 1; 271 (44) :27670-6. 4. Ladner RD, Caradonna SJ.The human dUTPase gene encodes both nuclear and mitochondrial isoforms.Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. J Biol Chem. 1997 Jul 25; 272 (30) :19072-80. 5. Ladner RD.The role of dUTPase and uracil-DNA repair in cancer chemotherapy. Curr Protein Pept Sci. 2001 Dec; 2 (4) :361-70.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

27 kDa

Observed Molecular Weight

18 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Subcellular Location

Isoform 2: Nucleus.

Sequence Similarities

Belongs to the dUTPase family.

Protein Name

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.