Anti-Lamin B1/LMNB1 Antibody Picoband®

About this product

Boster Bio Anti-Lamin B1/LMNB1 Antibody catalog # PA1048. Tested in IP, ICC/IF, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1048

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Synonyms

Lamin-B1; LMNB1; LMN2, LMNB

Gene Name

LMNB1

Gene ID

4001

UniProt

P20700

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1, different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed in non-excitable tissues.

Applications

IF, ICC/IF, IHC, WB

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Death, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Nucleus, Signal Transduction, Subcellular Markers, Tags & Cell Markers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

References & Citations

1. Pollard, K. M.; Chan, E. K.; Grant, B. J.; Sullivan, K. E.; Tan, E. M.; Glass, C. A. : In vitro posttranslational modification of lamin B clones from a human T-cell line. Molec. Cell. Biol. 10: 2164-2175, 1990 2. Tsai, M.-Y.; Wang, S.; Heidinger, J. M.; Shumaker, D. K.; Adam, S. A.; Goldman, R. D.; Zheng, Y. : A mitotic lamin B matrix induced by RanGTP required for spindle assembly. Science 311: 1887-1893, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

66 kDa

Observed Molecular Weight

70-72 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Lamin-B1

Subcellular Location

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.

Sequence Similarities

Belongs to the intermediate filament family.

Protein Name

Lamin-B1

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.