Anti-SKP2 Antibody Picoband®

About this product

Boster Bio Anti-SKP2 Antibody catalog # PA1102. Tested in Flow Cytometry, ICC/IF, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1102

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G (1) -S transition by controlling the stability of several G (1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.

Synonyms

S-phase kinase-associated protein 2; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2; SKP2; FBXL1

Gene Name

SKP2

Gene ID

6502

UniProt

Q13309

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SKP2, different from the related rat and mouse sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. .

Applications

Flow Cytometry, ICC/IF, WB

Field of Research

Cancer, Cardiogenesis, Cardiovascular, Cell Biology, F Box E3 Ligase, Oncoproteins, Oncoproteins/Suppressors, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Transcription Factors/Regulators, Ubiquitin E3 Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration. .

References & Citations

1. Kitagawa, M.; Lee, S. H.; McCormick, F. : Skp2 suppresses p53-dependent apoptosis by inhibiting p300. Molec. Cell 29: 217-231, 2008. 2. Latres, E.; Chiarle, R.; Schulman, B. A.; Pavletich, N. P.; Pellicer, A.; Inghirami, G.; Pagano, M. : Role of the F-box protein Skp2 in lymphomagenesis. Proc. Nat. Acad. Sci. 98: 2515-2520, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

48 kDa

Observed Molecular Weight

48 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

S-phase kinase-associated protein 2

Subcellular Location

Cytoplasm . Nucleus .

Sequence Similarities

Contains 1 F-box domain.

Protein Name

S-phase kinase-associated protein 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.