Anti-SHP2/PTPN11 Antibody Picoband®

About this product

Boster Bio Anti-SHP2/PTPN11 Antibody catalog # PA1114. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1114

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The tyrosine phosphatase Shp2 is recruited into tyrosine-kinase signalling pathways through binding of its two amino-terminal SH2 domains to specific phosphotyrosine motifs, concurrent with its re-localization and stimulation of phosphatase activity. Shp2 can potentiate signalling through the MAP-kinase pathway and is required during early mouse development for gastrulation. Shp2 is specifically required in mesenchyme cells of the progress zone (PZ), ly beneath the distal ectoderm of the limb bud. Rather than integrating proliferative signals, Shp2 probably exerts its effects on limb development by influencing cell shape, movement or adhesion. Furthermore, the branchial arches, which also use Fgfs during bud outgrowth, similarly require Shp2. Thus, Shp2 regulates phosphotyrosine-signalling events during the complex ectodermal-mesenchymal interactions that regulate mammalian budding morphogenesis.

Synonyms

Tyrosine-protein phosphatase non-receptor type 11;3.1.3.48; Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3; PTPN11; PTP2C, SHPTP2

Gene Name

PTPN11

Gene ID

5781

UniProt

Q06124

Host

Rabbit

Reactivity

Rat, Mouse, Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SHP2, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with highest levels in heart, brain, and skeletal muscle. .

Applications

IHC, WB

Field of Research

Auditory System, Neuroscience, Protein Phosphorylation, Sensory System, Signal Transduction, Tyrosine Phosphatases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity. .

References & Citations

1. Saxton, T. M.; Ciruna, B. G.; Holmyard, D.; Kulkarni, S.; Harpal, K.; Rossant, J.; Pawson, T. : The SH2 tyrosine phosphatase Shp2 is required for mammalian limb development. Nature Genet. 24: 420-423, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68436 MW

Observed Molecular Weight

70-74 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Tyrosine-protein phosphatase non-receptor type 11

Subcellular Location

Cytoplasm.

Sequence Similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

Protein Name

Tyrosine-protein phosphatase non-receptor type 11

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.