Anti-Cytochrome C/CYCS Antibody Picoband®

About this product

Boster Bio Anti-Cytochrome C/CYCS Antibody catalog # PA1118. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1118

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytochrome C is located in the mitochondria of all aerobic cells and is involved in the electron transport system. Human cytochrome c has 104 amino acid residues and a molecular weight of 11,458 and is mapped to 7p15.2. Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress. And it has a role in different apoptotic signaling cascades.

Synonyms

Cytochrome c; CYCS; CYC

Gene Name

CYCS

Gene ID

54205

UniProt

P99999

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with highest levels in heart, brain, and skeletal muscle. .

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Cancer Metabolism, Cardiovascular, Cell Biology, Cell Death, Cytochromes, Energy Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Invasion/Microenvironment, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oxidative Phosphorylation, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

References & Citations

1. Li, K.; Li, Y.; Shelton, J. M.; Richardson, J. A.; Spencer, E.; Chen, Z. J.; Wang, X.; Williams, R. S. : Cytochrome c deficiency causes embryonic lethality and attenuates stress-induced apoptosis. Cell 101: 389-399, 2000.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

11749 MW

Observed Molecular Weight

14 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cytochrome c

Subcellular Location

Mitochondrion intermembrane space. Loosely associated with the inner membrane.

Sequence Similarities

Belongs to the cytochrome c family.

Protein Name

Cytochrome c

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.