Anti-Topoisomerase II alpha/TOP2A Antibody Picoband®

About this product

Boster Bio Anti-Topoisomerase II alpha/TOP2A Antibody catalog # PA1127. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1127

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.

Synonyms

DNA topoisomerase 2-alpha;5.99.1.3; DNA topoisomerase II, alpha isozyme; TOP2A; TOP2

Gene Name

TOP2A

Gene ID

7153

UniProt

P11388

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha, different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids.

Clonality

Polyclonal

Applications

WB

Field of Research

Cancer, Chromosome Structure, DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity) . .

References & Citations

1. Miller, K. G.; Liu, L. F.; Englund, P. T. : A homogeneous type II DNA topoisomerase from HeLa cell nuclei. J. Biol. Chem. 256: 9334-9339, 1981. 2. Mondal, N.; Parvin, J. D. : DNA topoisomerase II-alpha is required for RNA polymerase II transcription on chromatin templates. Nature 413: 435-438, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

174385 MW

Observed Molecular Weight

174 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

DNA topoisomerase 2-alpha

Subcellular Location

Cytoplasm . Nucleus, nucleoplasm . Generally located in the nucleoplasm.

Sequence Similarities

Belongs to the type II topoisomerase family.

Protein Name

DNA topoisomerase 2-alpha

Isotype

Rabbit IgG

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.